7ckk

X-ray diffraction
2.35Å resolution

Structural complex of FTO bound with Dac51

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
N(6)-methyladenosine in mRNA + 2-oxoglutarate + O(2) = adenosine in mRNA + formaldehyde + succinate + CO(2)

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-189888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-ketoglutarate-dependent dioxygenase FTO Chain: A
Molecule details ›
Chain: A
Length: 473 amino acids
Theoretical weight: 54.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q9C0B1 (Residues: 32-500; Coverage: 93%)
  • Best match: Q9C0B1-4 (Residues: 1-101)
Gene names: FTO, KIAA1752
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R3
Unit cell:
a: 142.124Å b: 142.124Å c: 84.539Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.221 0.264
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'