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Crystal structures of aconitase X enzymes from bacteria and archaea provide insights into the molecular evolution of the aconitase superfamily.

Watanabe S, Murase Y, Watanabe Y, Sakurai Y, Tajima K
Commun Biol 4 687 (2021)
Related entries: 7cnp, 7cnr, 7cns, 7d2r

Cited: 6 times
EuropePMC logo PMID: 34099860

Abstract

Aconitase superfamily members catalyze the homologous isomerization of specific substrates by sequential dehydration and hydration and contain a [4Fe-4S] cluster. However, monomeric and heterodimeric types of function unknown aconitase X (AcnX) have recently been characterized as a cis-3-hydroxy-L-proline dehydratase (AcnXType-I) and mevalonate 5-phosphate dehydratase (AcnXType-II), respectively. We herein elucidated the crystal structures of AcnXType-I from Agrobacterium tumefaciens (AtAcnX) and AcnXType-II from Thermococcus kodakarensis (TkAcnX) without a ligand and in complex with substrates. AtAcnX and TkAcnX contained the [2Fe-2S] and [3Fe-4S] clusters, respectively, conforming to UV and EPR spectroscopy analyses. The binding sites of the [Fe-S] cluster and substrate were clearlydifferent from those that were completely conserved in other aconitase enzymes; however, theoverall structural frameworks and locations of active sites were partially similar to each other.These results provide novel insights into the evolutionary scenario of the aconitase superfamilybased on the recruitment hypothesis.

Articles - 7cnq mentioned but not cited (2)

  1. Crystal structures of aconitase X enzymes from bacteria and archaea provide insights into the molecular evolution of the aconitase superfamily. Watanabe S, Murase Y, Watanabe Y, Sakurai Y, Tajima K. Commun Biol 4 687 (2021)
  2. A [4Fe-4S] cluster resides at the active center of phosphomevalonate dehydratase, a key enzyme in the archaeal modified mevalonate pathway. Komeyama M, Kanno K, Mino H, Yasuno Y, Shinada T, Ito T, Hemmi H. Front Microbiol 14 1150353 (2023)


Reviews citing this publication (1)

  1. The catalytic and structural basis of archaeal glycerophospholipid biosynthesis. de Kok NAW, Driessen AJM. Extremophiles 26 29 (2022)

Articles citing this publication (3)

  1. Genomic remnants of ancestral methanogenesis and hydrogenotrophy in Archaea drive anaerobic carbon cycling. Adam PS, Kolyfetis GE, Bornemann TLV, Vorgias CE, Probst AJ. Sci Adv 8 eabm9651 (2022)
  2. Unravelling the role of transient redox partner complexes in P450 electron transfer mechanics. Hargrove TY, Lamb DC, Smith JA, Wawrzak Z, Kelly SL, Lepesheva GI. Sci Rep 12 16232 (2022)
  3. Stabilisation of the RirA [4Fe-4S] cluster results in loss of iron-sensing function. Gray E, Stewart MYY, Hanwell L, Crack JC, Devine R, Stevenson CEM, Volbeda A, Johnston AWB, Fontecilla-Camps JC, Hutchings MI, Todd JD, Le Brun NE. Chem Sci 14 9744-9758 (2023)


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