› 7cov

X-ray diffraction
2Å resolution

Potato D-enzyme, native (substrate free)

Released:
DOI: 10.2210/pdb7cov/pdb
PDB entry 7cov coloured by chain, front view.
PDB entry 7cov coloured by chain, side view.
PDB entry 7cov coloured by chain, top view.
Source organism: Solanum tuberosum
Primary publication:
Structural analysis and reaction mechanism of the disproportionating enzyme (D-enzyme) from potato.
Imamura K, Matsuura T, Nakagawa A, Kitamura S, Kusunoki M, Takaha T, Unno H
Protein Sci 29 2085-2100 (2020)
PMID: 32808707

Function and Biology Details

Reaction catalysed: 
Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170396 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-alpha-glucanotransferase, chloroplastic/amyloplastic Chain: A
Molecule details ›
Chain: A
Length: 576 amino acids
Theoretical weight: 65.02 KDa
Source organism: Solanum tuberosum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06801 (Residues: 1-576; Coverage: 100%)
Gene name: DPEP
Sequence domains: 4-alpha-glucanotransferase

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2221
Unit cell:
a: 69.869Å b: 120.535Å c: 174.381Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.188
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Production of Large-Ring Cyclodextrins by Amylomaltases.
Krusong et al. (2022)
1 more

1 mention without citation

Structural analysis and reaction mechanism of the disproportionating enzyme (D-enzyme) from potato.
Imamura et al. (2020)