› 7ctx

X-ray diffraction
2.91Å resolution

Crystal structure of Arabidopsis thaliana SOBIR1 kinase domain(residues 388-401 deleted) in complex with AMP-PNP and magnesium

Released:
DOI: 10.2210/pdb7ctx/pdb
PDB entry 7ctx coloured by chain, front view.
PDB entry 7ctx coloured by chain, side view.
PDB entry 7ctx coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Wei X, Wang YL, Gu TY, Xin FJ

Function and Biology Details

Reactions catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine-rich repeat receptor-like serine/threonine/tyrosine-protein kinase SOBIR1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 36.27 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SKB2 (Residues: 326-640; Coverage: 50%)
Gene names: At2g31880, EVR, F20M17.8, SOBIR1
Sequence domains: Protein kinase domain

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 72.389Å b: 50.732Å c: 93.066Å
α: 90° β: 106.65° γ: 90°
R-values:
R R work R free
0.226 0.225 0.244
Expression system: Escherichia coli BL21(DE3)

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