› 7cun

Electron Microscopy
3.5Å resolution

The structure of human Integrator-PP2A complex

Released:
DOI: 10.2210/pdb7cun/pdb
PDB entry 7cun coloured by chain, front view.
PDB entry 7cun coloured by chain, side view.
PDB entry 7cun coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.
Zheng H, Qi Y, Hu S, Cao X, Xu C, Yin Z, Chen X, Li Y, Liu W, Li J, Wang J, Wei G, Liang K, Chen FX, Xu Y
Science 370 (2020)
PMID: 33243860
Related structures: EMD-30473

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-151662 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (12 distinct):
Integrator complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 2190 amino acids
Theoretical weight: 244.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8N201 (Residues: 1-2190; Coverage: 100%)
Gene names: INTS1, KIAA1440, UNQ1821/PRO3434
Sequence domains:
Integrator complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 1204 amino acids
Theoretical weight: 134.45 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9H0H0 (Residues: 1-1204; Coverage: 100%)
Gene names: INTS2, KIAA1287
Sequence domains: Integrator complex subunit 2
Integrator complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 963 amino acids
Theoretical weight: 108.31 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96HW7 (Residues: 1-963; Coverage: 100%)
Gene names: INTS4, MSTP093
Sequence domains:
Integrator complex subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 1019 amino acids
Theoretical weight: 108.12 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6P9B9 (Residues: 1-1019; Coverage: 100%)
Gene names: INTS5, KIAA1698
Sequence domains:
Integrator complex subunit 6 Chain: F
Molecule details ›
Chain: F
Length: 887 amino acids
Theoretical weight: 100.53 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UL03 (Residues: 1-887; Coverage: 100%)
Gene names: DBI1, DDX26, DDX26A, INTS6
Sequence domains:
Integrator complex subunit 7 Chain: G
Molecule details ›
Chain: G
Length: 962 amino acids
Theoretical weight: 106.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NVH2 (Residues: 1-962; Coverage: 100%)
Gene names: C1orf73, INTS7
Sequence domains: Integrator complex subunit 7, C-terminal domain
Integrator complex subunit 8 Chain: H
Molecule details ›
Chain: H
Length: 995 amino acids
Theoretical weight: 113.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q75QN2 (Residues: 1-995; Coverage: 100%)
Gene names: C8orf52, INTS8
Integrator complex subunit 9 Chain: I
Molecule details ›
Chain: I
Length: 658 amino acids
Theoretical weight: 73.89 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NV88 (Residues: 1-658; Coverage: 100%)
Gene names: INTS9, RC74
Sequence domains:
Integrator complex subunit 11 Chain: K
Molecule details ›
Chain: K
Length: 600 amino acids
Theoretical weight: 67.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5TA45 (Residues: 1-600; Coverage: 100%)
Gene names: CPSF3L, INTS11, RC68
Sequence domains:
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Chain: P
Molecule details ›
Chain: P
Length: 589 amino acids
Theoretical weight: 66.03 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P30153 (Residues: 1-589; Coverage: 100%)
Gene name: PPP2R1A
Sequence domains: HEAT repeat
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Chain: Q
Molecule details ›
Chain: Q
Length: 309 amino acids
Theoretical weight: 35.64 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P67775 (Residues: 1-309; Coverage: 100%)
Gene name: PPP2CA
Sequence domains: Calcineurin-like phosphoesterase
unknown Chain: U
Molecule details ›
Chain: U
Length: 594 amino acids
Theoretical weight: 50.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand MN 2 x MN
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-30473
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

14 review citations

Targeting transcription cycles in cancer.
Vervoort et al. (2022)
13 more

4 mentions without citation

Advancements in chemical biology targeting the kinases and phosphatases of RNA polymerase II-mediated transcription.
Kim et al. (2021)
3 more