7cwh

Solution NMR

Structural basis of RACK7 PHD to read a pediatric glioblastoma-associated histone mutation H3.3G34R

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-160870 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone H3.3 Chain: A
Molecule details ›
Chain: A
Length: 11 amino acids
Theoretical weight: 1.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P84243 (Residues: 32-42; Coverage: 8%)
Gene names: H3-3A, H3-3B, H3.3A, H3.3B, H3F3, H3F3A, H3F3B, PP781
MYND-type zinc finger-containing chromatin reader ZMYND8 Chain: B
Molecule details ›
Chain: B
Length: 61 amino acids
Theoretical weight: 7.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ULU4 (Residues: 83-143; Coverage: 5%)
Gene names: KIAA1125, PRKCBP1, RACK7, ZMYND8
Sequence domains: PHD-finger

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment: 85%
Refinement method: simulated annealing
Expression systems:
  • Not provided
  • Escherichia coli