› 7d1h

X-ray diffraction
1.69Å resolution

Crystal structure of Ixodes scapularis glutaminyl cyclase with D238A mutation

Released:
DOI: 10.2210/pdb7d1h/pdb
PDB entry 7d1h coloured by chain, front view.
PDB entry 7d1h coloured by chain, side view.
PDB entry 7d1h coloured by chain, top view.
Source organism: Ixodes scapularis
Primary publication:
A Unique Carboxylic-Acid Hydrogen-Bond Network (CAHBN) Confers Glutaminyl Cyclase Activity on M28 Family Enzymes.
Huang KF, Huang JS, Wu ML, Hsieh WL, Hsu KC, Hsu HL, Ko TP, Wang AH
J Mol Biol 433 166960 (2021)
PMID: 33774034

Function and Biology Details

Reaction catalysed: 
L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminyl-peptide cyclotransferase Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 38.25 KDa
Source organism: Ixodes scapularis
Expression system: Escherichia coli
UniProt:
  • Canonical: B7QK46 (Residues: 25-353; Coverage: 99%)
Gene names: IscW_ISCW023264, Qptc
Sequence domains: Peptidase family M28

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P212121
Unit cell:
a: 55.534Å b: 71.403Å c: 80.225Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.218
Expression system: Escherichia coli

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Citations

1 citations in other articles

Helium Nanodroplet Infrared Action Spectroscopy of the Proton-Bound Dimer of Hydrogen Sulfate and Formate: Examining Nuclear Quantum Effects.
Thomas et al. (2021)