› 7d2a

X-ray diffraction
1.57Å resolution

CBM32 of AlyQ in complex with 4,5-unsaturated mannuronic acid

Released:
DOI: 10.2210/pdb7d2a/pdb
PDB entry 7d2a coloured by chain, front view.
PDB entry 7d2a coloured by chain, side view.
PDB entry 7d2a coloured by chain, top view.
Source organism: Persicobacter sp. CCB-QB2
Primary publication:
Structural basis for binding uronic acids by family 32 carbohydrate-binding modules.
Teh AH, Sim PF, Hisano T
Biochem Biophys Res Commun 533 257-261 (2020)
PMID: 33010888

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104803 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
F5/8 type C domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.1 KDa
Source organism: Persicobacter sp. CCB-QB2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A3B6UEP6 (Residues: 1-143; Coverage: 36%)
Sequence domains: F5/8 type C domain

Ligands and Environments

Carbohydrate polymer : NEW Components: LGU, MAW
Carbohydrate polymer
3 bound ligands:
Ligand CA 1 x CA
Ligand PG4 1 x PG4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 43.608Å b: 55.773Å c: 58.427Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.194
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

A bacterial sulfoglycosidase highlights mucin O-glycan breakdown in the gut ecosystem.
Katoh et al. (2023)
2 more