7d3s

Electron Microscopy
2.9Å resolution

Human SECR in complex with an engineered Gs heterotrimer

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-209415 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Secretin Chain: P
Molecule details ›
Chain: P
Length: 27 amino acids
Theoretical weight: 3.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P09683 (Residues: 28-54; Coverage: 26%)
Gene name: SCT
Sequence domains: Peptide hormone
Secretin receptor Chain: R
Molecule details ›
Chain: R
Length: 434 amino acids
Theoretical weight: 49.74 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P47872 (Residues: 23-440; Coverage: 100%)
Gene name: SCTR
Sequence domains:
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63092 (Residues: 5-394; Coverage: 96%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 68 amino acids
Theoretical weight: 7.55 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-67; Coverage: 94%)
Gene name: GNG2
Sequence domains: GGL domain
nanobody Nb35 Chain: N
Molecule details ›
Chain: N
Length: 137 amino acids
Theoretical weight: 15.02 KDa
Source organism: unidentified
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-30566
Expression systems:
  • Not provided
  • Spodoptera frugiperda
  • Escherichia coli