› 7d3s

Electron Microscopy
2.9Å resolution

Human SECR in complex with an engineered Gs heterotrimer

Released:
DOI: 10.2210/pdb7d3s/pdb
PDB entry 7d3s coloured by chain, front view.
PDB entry 7d3s coloured by chain, side view.
PDB entry 7d3s coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the human secretin receptor coupled to an engineered heterotrimeric G protein.
Fukuhara S, Kobayashi K, Kusakizako T, Iida W, Kato M, Shihoya W, Nureki O
Biochem Biophys Res Commun 533 861-866 (2020)
PMID: 33008599
Related structures: EMD-30566

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-209415 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Secretin Chain: P
Molecule details ›
Chain: P
Length: 27 amino acids
Theoretical weight: 3.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
Secretin receptor Chain: R
Molecule details ›
Chain: R
Length: 434 amino acids
Theoretical weight: 49.74 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 68 amino acids
Theoretical weight: 7.55 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
nanobody Nb35 Chain: N
Molecule details ›
Chain: N
Length: 137 amino acids
Theoretical weight: 15.02 KDa
Source organism: unidentified
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-30566
Expression systems:
  • Not provided
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 review citation

Intestinal Enteroendocrine Cells: Present and Future Druggable Targets.
Atanga et al. (2023)
6 other articles

3 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
2 more