› 7d53

X-ray diffraction
1.6Å resolution

SpuA mutant - H221N with Glu

Released:
DOI: 10.2210/pdb7d53/pdb
PDB entry 7d53 coloured by chain, front view.
PDB entry 7d53 coloured by chain, side view.
PDB entry 7d53 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structure and mechanism of the γ-glutamyl-γ-aminobutyrate hydrolase SpuA from Pseudomonas aeruginosa.
Chen Y, Jia H, Zhang J, Liang Y, Liu R, Zhang Q, Bartlam M
Acta Crystallogr D Struct Biol 77 1305-1316 (2021)
PMID: 34605433

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable glutamine amidotransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 250 amino acids
Theoretical weight: 26.48 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I6J4 (Residues: 1-250; Coverage: 100%)
Gene names: PA0297, spuA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand GLU 4 x GLU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 47.349Å b: 120.378Å c: 158.786Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.163 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Discovery of a glutathione utilization pathway in Francisella that shows functional divergence between environmental and pathogenic species.
Wang et al. (2023)