› 7d76

Electron Microscopy
3.1Å resolution

Cryo-EM structure of the beclomethasone-bound adhesion receptor GPR97-Go complex

Released:
DOI: 10.2210/pdb7d76/pdb
PDB entry 7d76 coloured by chain, front view.
PDB entry 7d76 coloured by chain, side view.
PDB entry 7d76 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the glucocorticoid-bound adhesion receptor GPR97-Go complex.
Ping YQ, Mao C, Xiao P, Zhao RJ, Jiang Y, Yang Z, An WT, Shen DD, Yang F, Zhang H, Qu C, Shen Q, Tian C, Li ZJ, Li S, Wang GY, Tao X, Wen X, Zhong YN, Yang J, Yi F, Yu X, Xu HE, Zhang Y, Sun JP
Nature 589 620-626 (2021)
PMID: 33408414
Related structures: EMD-30602

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-140669 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Guanine nucleotide-binding protein G(o) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 226 amino acids
Theoretical weight: 25.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P09471 (Residues: 4-354; Coverage: 64%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 346 amino acids
Theoretical weight: 38.25 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Adhesion G protein-coupled receptor G3 Chain: R
Molecule details ›
Chain: R
Length: 561 amino acids
Theoretical weight: 62.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q86Y34 (Residues: 14-549; Coverage: 100%)
Gene names: ADGRG3, GPR97, PGR26
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand PLM 3 x PLM
Ligand Y01 1 x Y01
Ligand GXR 1 x GXR
Ligand CLR 2 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-30602
Expression system: Spodoptera frugiperda

Quick links

Citations

15 review citations

G protein-coupled receptor signaling: transducers and effectors.
Jiang et al. (2022)
14 more

7 mentions without citation

The Novel Application of Geometric Morphometrics with Principal Component Analysis to Existing G Protein-Coupled Receptor (GPCR) Structures.
Wiseman et al. (2021)
6 more