› 7d77

Electron Microscopy
2.9Å resolution

Cryo-EM structure of the cortisol-bound adhesion receptor GPR97-Go complex

Released:
DOI: 10.2210/pdb7d77/pdb
PDB entry 7d77 coloured by chain, front view.
PDB entry 7d77 coloured by chain, side view.
PDB entry 7d77 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of the glucocorticoid-bound adhesion receptor GPR97-Go complex.
Ping YQ, Mao C, Xiao P, Zhao RJ, Jiang Y, Yang Z, An WT, Shen DD, Yang F, Zhang H, Qu C, Shen Q, Tian C, Li ZJ, Li S, Wang GY, Tao X, Wen X, Zhong YN, Yang J, Yi F, Yu X, Xu HE, Zhang Y, Sun JP
Nature 589 620-626 (2021)
PMID: 33408414
Related structures: EMD-30603

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-209411 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(o) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 226 amino acids
Theoretical weight: 25.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P09471 (Residues: 4-354; Coverage: 64%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 346 amino acids
Theoretical weight: 38.25 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Adhesion G protein-coupled receptor G3 Chain: R
Molecule details ›
Chain: R
Length: 561 amino acids
Theoretical weight: 62.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q86Y34 (Residues: 14-549; Coverage: 100%)
Gene names: ADGRG3, GPR97, PGR26
Sequence domains:
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 250 amino acids
Theoretical weight: 26.61 KDa
Source organism: synthetic construct
Expression system: Spodoptera frugiperda

Ligands and Environments

4 bound ligands:
Ligand PLM 3 x PLM
Ligand Y01 1 x Y01
Ligand HCY 1 x HCY
Ligand CLR 2 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-30603
Expression system: Spodoptera frugiperda

Quick links

Citations

15 review citations

Structure determination of GPCRs: cryo-EM compared with X-ray crystallography.
García-Nafría et al. (2021)
14 more

9 mentions without citation

Structure, function and drug discovery of GPCR signaling.
Cheng et al. (2023)
8 more