› 7d7m

Electron Microscopy
3.3Å resolution

Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein

Released:
DOI: 10.2210/pdb7d7m/pdb
PDB entry 7d7m coloured by chain, front view.
PDB entry 7d7m coloured by chain, side view.
PDB entry 7d7m coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein.
Nojima S, Fujita Y, Kimura KT, Nomura N, Suno R, Morimoto K, Yamamoto M, Noda T, Iwata S, Shigematsu H, Kobayashi T
Structure 29 252-260.e6 (2021)
PMID: 33264604
Related structures: EMD-30608

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-211102 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Prostaglandin E2 receptor EP4 subtype Chain: A
Molecule details ›
Chain: A
Length: 333 amino acids
Theoretical weight: 36.95 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P35408 (Residues: 4-366; Coverage: 66%)
Gene names: PTGER2, PTGER4
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short; Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: D
Molecule details ›
Chain: D
Length: 249 amino acids
Theoretical weight: 29.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63092 (Residues: 5-64, 204-394; Coverage: 61%)
  • Best match: P63092-2 (Residues: 5-64, 190-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
nanobody Nb35 Chain: E
Molecule details ›
Chain: E
Length: 134 amino acids
Theoretical weight: 14.68 KDa
Source organism: Lama glama
Expression system: Brevibacillus choshinensis

Ligands and Environments

1 bound ligand:
Ligand P2E 1 x P2E
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-30608
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)
  • Brevibacillus choshinensis

Quick links

Citations

1 review citation

Ligands of Adrenergic Receptors: A Structural Point of View.
Wu et al. (2021)
10 other articles

10 mentions without citation

Allosteric communication regulates ligand-specific GPCR activity.
Ma et al. (2021)
9 more