› 7d8u

X-ray diffraction
2.7Å resolution

Crystal structure of the C-terminal domain of pNP868R from African swine fever virus

Released:
DOI: 10.2210/pdb7d8u/pdb
PDB entry 7d8u coloured by chain, front view.
PDB entry 7d8u coloured by chain, side view.
PDB entry 7d8u coloured by chain, top view.
Source organism: African swine fever virus
Primary publication:
Structure and Biochemical Characteristic of the Methyltransferase (MTase) Domain of RNA Capping Enzyme from African Swine Fever Virus.
Du X, Gao ZQ, Geng Z, Dong YH, Zhang H
J Virol (2020)
PMID: 33268516

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
A 5'-triphospho-[mRNA] + H(2)O = a 5'-diphospho-[mRNA] + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA-capping enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 33.06 KDa
Source organism: African swine fever virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32094 (Residues: 586-868; Coverage: 33%)
Gene names: Ba71V-101, NP868R
Sequence domains: mRNA (guanine-N(7))-methyltransferase domain

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
2 bound ligands:
Ligand EDO 7 x EDO
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P6522
Unit cell:
a: 127.315Å b: 127.315Å c: 208.198Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.184 0.226
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structure of African Swine Fever Virus and Associated Molecular Mechanisms Underlying Infection and Immunosuppression: A Review.
Wang et al. (2021)
2 more

3 mentions without citation

Structure and Biochemical Characteristic of the Methyltransferase (MTase) Domain of RNA Capping Enzyme from African Swine Fever Virus.
Du et al. (2021)
2 more