› 7day

X-ray diffraction
1.48Å resolution

Crystal structure of Drosophila melanogaster Noppera-bo, glutathione S-transferase epsilon 14 (DmGSTE14), in TDP013-, and GSH-bound form

Released:
DOI: 10.2210/pdb7day/pdb
PDB entry 7day coloured by chain, front view.
PDB entry 7day coloured by chain, side view.
PDB entry 7day coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Non-steroidal inhibitors of Drosophila melanogaster steroidogenic glutathione S -transferase Noppera-bo
Koiwai K, Morohashi K, Inaba K, Ebihara K, Kojima H, Okabe T, Yoshino R, Hirokawa T, Nampo T, Fujikawa Y, Inoue H, Yumoto F, Senda T, Niwa R
J Pestic Sci 46 75-87 (2021)

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181526 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase E14 Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 27.54 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7JYX0 (Residues: 1-232; Coverage: 100%)
Gene names: CG4688, GSTD14-14, GstE14, nobo
Sequence domains:

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH
2 bound ligands:
Ligand H1X 3 x H1X
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 58.7Å b: 75.1Å c: 108.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.204
Expression system: Escherichia coli BL21(DE3)

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