› 7db9

X-ray diffraction
2.84Å resolution

IC1 in complex with tubulin

Released:
DOI: 10.2210/pdb7db9/pdb
PDB entry 7db9 coloured by chain, front view.
PDB entry 7db9 coloured by chain, side view.
PDB entry 7db9 coloured by chain, top view.
Source organisms:
Entry authors: Wu CY, Wang YX

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-236943 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Tubulin beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 143 amino acids
Theoretical weight: 16.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63042 (Residues: 49-189; Coverage: 75%)
Gene name: Stmn4
Sequence domains: Stathmin family
Tubulin tyrosine ligase Chain: F
Molecule details ›
Chain: F
Length: 384 amino acids
Theoretical weight: 44.38 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli

Ligands and Environments

7 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 5 x MG
Ligand CA 4 x CA
Ligand GDP 2 x GDP
Ligand MES 2 x MES
Ligand IC1 2 x IC1
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 105.124Å b: 157.684Å c: 182.116Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.233
Expression system: Escherichia coli

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