› 7dbe

X-ray diffraction
1.9Å resolution

Structure of a novel transaminase

Released:
DOI: 10.2210/pdb7dbe/pdb
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PDB entry 7dbe coloured by chain, front view.
PDB entry 7dbe coloured by chain, side view.
PDB entry 7dbe coloured by chain, top view.
Source organism: Rhodobacter sp. 140A
Primary publication:
Biochemical and Structural Characterization of an (R)-Selective Transaminase in the Asymmetric Synthesis of Chiral Hydroxy Amines.
Li FL, Liang YX, Wei YW, Zheng YK, Yu HM
Adv. Synth. Catal. (2021)

Function and Biology Details

Reaction catalysed: 
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable branched-chain-amino-acid aminotransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 341 amino acids
Theoretical weight: 37.68 KDa
Source organism: Rhodobacter sp. 140A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A8M0FGQ9 (Residues: 1-334; Coverage: 100%)
Sequence domains: Amino-transferase class IV

Ligands and Environments


Cofactor: Ligand PLP 4 x PLP
1 bound ligand:
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 62.267Å b: 141.074Å c: 90.755Å
α: 90° β: 100.111° γ: 90°
R-values:
R R work R free
0.162 0.16 0.201
Expression system: Escherichia coli BL21(DE3)

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