› 7ddb

X-ray diffraction
1.72Å resolution

Crystal structure of fungal antifreeze protein with intermediate activity

Released:
DOI: 10.2210/pdb7ddb/pdb
PDB entry 7ddb coloured by chain, front view.
PDB entry 7ddb coloured by chain, side view.
PDB entry 7ddb coloured by chain, top view.
Source organism: Typhula ishikariensis
Primary publication:
Characterization of microbial antifreeze protein with intermediate activity suggests that a bound-water network is essential for hyperactivity.
Khan NMU, Arai T, Tsuda S, Kondo H
OpenAccess logo Sci Rep 11 5971 (2021)
PMID: 33727595

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antifreeze protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 223 amino acids
Theoretical weight: 22.09 KDa
Source organism: Typhula ishikariensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q76CE7 (Residues: 21-243; Coverage: 100%)
Gene name: K1-C
Sequence domains: Ice-binding-like

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P212121
Unit cell:
a: 56.79Å b: 63.143Å c: 102.086Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Cold Adaptation Strategies and the Potential of Psychrophilic Enzymes from the Antarctic Yeast, Glaciozyma antarctica PI12.
Yusof et al. (2021)
3 more