7dfa

X-ray diffraction
2.54Å resolution

Crystal of Arrestin2-V2Rpp-4-Fab30 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-210715 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Beta-arrestin-1 Chain: A
Molecule details ›
Chain: A
Length: 426 amino acids
Theoretical weight: 48.27 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P17870 (Residues: 1-418; Coverage: 100%)
Gene name: ARRB1
Sequence domains:
FAB30 HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 249 amino acids
Theoretical weight: 26.83 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
FAB30 LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 227 amino acids
Theoretical weight: 24.75 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Vasopressin V2 receptor Chain: V
Molecule details ›
Chain: V
Length: 23 amino acids
Theoretical weight: 2.91 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P30518 (Residues: 346-368; Coverage: 6%)
Gene names: ADHR, AVPR2, DIR, DIR3, V2R

Ligands and Environments

No bound ligands
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: I212121
Unit cell:
a: 116.901Å b: 121.142Å c: 144.504Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.234 0.28
Expression systems:
  • Escherichia coli
  • Not provided