› 7dhr

Electron Microscopy
3.8Å resolution

Cryo-EM structure of the full agonist isoprenaline-bound beta2 adrenergic receptor-Gs protein complex.

Released:
DOI: 10.2210/pdb7dhr/pdb
PDB entry 7dhr coloured by chain, front view.
PDB entry 7dhr coloured by chain, side view.
PDB entry 7dhr coloured by chain, top view.
Source organisms:
Primary publication:
Different Conformational Responses of the beta2-Adrenergic Receptor-Gs Complex upon Binding of the Partial Agonist Salbutamol or the Full Agonist Isoprenaline
Yang F, Ling SL, Zhou YX, Zhang YN, Lv P, Liu SL, Fang W, Sun WJ, Hu LYA, Zhang L, Shi P, Tian C
Natl Sci Rev (2020)
Related structures: EMD-30682

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-209314 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Nb35 nanobody Chain: N
Beta-2 adrenergic receptor Chain: R

Ligands and Environments

1 bound ligand:
Ligand 5FW 1 x 5FW
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-30682
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

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