› 7dhr

Electron Microscopy
3.8Å resolution

Cryo-EM structure of the full agonist isoprenaline-bound beta2 adrenergic receptor-Gs protein complex.

Released:
DOI: 10.2210/pdb7dhr/pdb
PDB entry 7dhr coloured by chain, front view.
PDB entry 7dhr coloured by chain, side view.
PDB entry 7dhr coloured by chain, top view.
Source organisms:
Primary publication:
Different Conformational Responses of the beta2-Adrenergic Receptor-Gs Complex upon Binding of the Partial Agonist Salbutamol or the Full Agonist Isoprenaline
Yang F, Ling SL, Zhou YX, Zhang YN, Lv P, Liu SL, Fang W, Sun WJ, Hu LYA, Zhang L, Shi P, Tian C
Natl Sci Rev (2020)
Related structures: EMD-30682

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-209314 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
  • Best match: P63092-4 (Residues: 1-395)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P62871 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 78 amino acids
Theoretical weight: 8.81 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nb35 nanobody Chain: N
Molecule details ›
Chain: N
Length: 157 amino acids
Theoretical weight: 17.06 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Beta-2 adrenergic receptor Chain: R
Molecule details ›
Chain: R
Length: 367 amino acids
Theoretical weight: 42.04 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07550 (Residues: 1-348; Coverage: 81%)
Gene names: ADRB2, ADRB2R, B2AR
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

1 bound ligand:
Ligand 5FW 1 x 5FW
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-30682
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

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