› 7dih

X-ray diffraction
1.5Å resolution

Crystal structure of Thermoglobin Y29F mutant in complex with imidazole

Released:
DOI: 10.2210/pdb7dih/pdb
PDB entry 7dih coloured by chain, front view.
PDB entry 7dih coloured by chain, side view.
PDB entry 7dih coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Structural Characterization of Y29F Mutant of Thermoglobin from a Hyperthermophilic Bacterium Aquifex aeolicus
Muraki N, Takeda K, Nam D, Muraki M, Aono S
Chem Lett. (2021)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130346 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized globin-like protein aq_211 Chains: A, B
Molecule details ›
Chains: A, B
Length: 142 amino acids
Theoretical weight: 16.25 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66586 (Residues: 1-139; Coverage: 100%)
Gene name: aq_211
Sequence domains: Globin

Ligands and Environments

3 bound ligands:
Ligand HEM 2 x HEM
Ligand IMD 2 x IMD
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Unit cell:
a: 59.771Å b: 35.823Å c: 138.084Å
α: 90° β: 95.2° γ: 90°
R-values:
R R work R free
0.2 0.199 0.223
Expression system: Escherichia coli BL21(DE3)

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