› 7dii

X-ray diffraction
2.4Å resolution

Crystal structure of LeuT in lipidic cubic phase at pH 7

Released:
DOI: 10.2210/pdb7dii/pdb
PDB entry 7dii coloured by chain, front view.
PDB entry 7dii coloured by chain, side view.
PDB entry 7dii coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Crystal structures of LeuT reveal conformational dynamics in the outward-facing states.
Fan J, Xiao Y, Quick M, Yang Y, Sun Z, Javitch JA, Zhou X
OpenAccess logo J Biol Chem 296 100609
PMID: 33811858

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+):neurotransmitter symporter (Snf family) Chains: A, B
Molecule details ›
Chains: A, B
Length: 513 amino acids
Theoretical weight: 57.45 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 1-513; Coverage: 100%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

2 bound ligands:
Ligand LEU 2 x LEU
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 64.588Å b: 72.184Å c: 80.806Å
α: 100.89° β: 97.58° γ: 115.16°
R-values:
R R work R free
0.193 0.192 0.213
Expression system: Escherichia coli

Quick links

Citations

1 review citation

General principles of secondary active transporter function.
Beckstein et al. (2022)
2 other articles

2 mentions without citation

Smad2 functions as a co-activator of canonical Wnt/beta-catenin signaling pathway independent of Smad4 through histone acetyltransferase activity of p300.
Hirota et al. (2008)
1 more