› 7djc

X-ray diffraction
2.7Å resolution

Crystal structure of the G26C/Q250A mutant of LeuT

Released:
DOI: 10.2210/pdb7djc/pdb
PDB entry 7djc coloured by chain, front view.
PDB entry 7djc coloured by chain, side view.
PDB entry 7djc coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Crystal structures of LeuT reveal conformational dynamics in the outward-facing states.
Fan J, Xiao Y, Quick M, Yang Y, Sun Z, Javitch JA, Zhou X
OpenAccess logo J Biol Chem 296 100609
PMID: 33811858

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+):neurotransmitter symporter (Snf family) Chain: A
Molecule details ›
Chain: A
Length: 513 amino acids
Theoretical weight: 57.44 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 1-513; Coverage: 100%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

3 bound ligands:
Ligand LEU 1 x LEU
Ligand NA 2 x NA
Ligand BOG 1 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C2
Unit cell:
a: 90.758Å b: 87.606Å c: 81.813Å
α: 90° β: 94.35° γ: 90°
R-values:
R R work R free
0.208 0.206 0.242
Expression system: Escherichia coli

Quick links

Citations

1 review citation

General principles of secondary active transporter function.
Beckstein et al. (2022)
2 other articles

1 mention without citation

Crystal structures of LeuT reveal conformational dynamics in the outward-facing states.
Fan et al. (2021)