› 7djq

X-ray diffraction
2.3Å resolution

Crystal Structure of O-acetyl L-serine sulfhydrylase from Haemophilus influenzae in complex with C-Terminal peptide of ribosomal S4 Domain protein from Lactobacillus salivarius.

Released:
DOI: 10.2210/pdb7djq/pdb
PDB entry 7djq coloured by chain, front view.
PDB entry 7djq coloured by chain, side view.
PDB entry 7djq coloured by chain, top view.
Source organisms:
Primary publication:
Moonlighting Biochemistry of Cysteine Synthase: A Species-specific Global Regulator.
Singh RP, Saini N, Sharma G, Rahisuddin R, Patel M, Kaushik A, Kumaran S
J Mol Biol 433 167255 (2021)
PMID: 34547327

Function and Biology Details

Reaction catalysed: 
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103822 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA-binding S4 domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.19 KDa
Source organism: Ligilactobacillus salivarius UCC118
Expression system: Not provided
UniProt:
  • Canonical: A0A1V9TQZ2 (Residues: 81-89; Coverage: 10%)
Gene name: B6U37_07150
Cysteine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 37.24 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P45040 (Residues: 1-316; Coverage: 100%)
Gene names: HI_1103, cysK
Sequence domains: Pyridoxal-phosphate dependent enzyme

Ligands and Environments

1 bound ligand:
Ligand NA 3 x NA
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 47.08Å b: 98.94Å c: 70.29Å
α: 90° β: 95.77° γ: 90°
R-values:
R R work R free
0.191 0.189 0.229
Expression systems:
  • Not provided
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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