› 7dni

Electron Microscopy
3.2Å resolution

MDA5 CARDs-MAVS CARD polyUb complex

Released:
DOI: 10.2210/pdb7dni/pdb
PDB entry 7dni coloured by chain, front view.
PDB entry 7dni coloured by chain, side view.
PDB entry 7dni coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ordered assembly of the cytosolic RNA-sensing MDA5-MAVS signaling complex via binding to unanchored K63-linked poly-ubiquitin chains.
Song B, Chen Y, Liu X, Yuan F, Tan EYJ, Lei Y, Song N, Han Y, Pascal BD, Griffin PR, Luo C, Wu B, Luo D, Zheng J
Immunity 54 2218-2230.e5 (2021)
PMID: 34644557
Related structures: EMD-30784

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
PDBe Complex ID:
PDB-CPX-143322 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin Chains: E, F, G, H, I, J, K, L
Molecule details ›
Chains: E, F, G, H, I, J, K, L
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family
Interferon-induced helicase C domain-containing protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 208 amino acids
Theoretical weight: 23.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BYX4 (Residues: 1-208; Coverage: 20%)
Gene names: IFIH1, MDA5, RH116
Sequence domains:
Mitochondrial antiviral-signaling protein Chains: M, N, O, P
Molecule details ›
Chains: M, N, O, P
Length: 97 amino acids
Theoretical weight: 11.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z434 (Residues: 1-97; Coverage: 18%)
Gene names: IPS1, KIAA1271, MAVS, VISA
Sequence domains: Caspase recruitment domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-30784
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Cytoplasmic RNA sensors and their interplay with RNA-binding partners in innate antiviral response: theme and variations.
Chan et al. (2022)
2 more