› 7dog

X-ray diffraction
2.91Å resolution

Crystal structure of a nuclease and capping domain of SbcD from Staphylococcus aureus

Released:
DOI: 10.2210/pdb7dog/pdb
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PDB entry 7dog coloured by chain, front view.
PDB entry 7dog coloured by chain, side view.
PDB entry 7dog coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
Crystal structure of the nuclease and capping domain of SbcD from Staphylococcus aureus.
Lee J, Jo I, Ahn J, Hong S, Jeong S, Kwon A, Ha NC
J Microbiol 59 584-589 (2021)
PMID: 33877576

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189398 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease SbcCD subunit D Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 36.82 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99UD1 (Residues: 2-320; Coverage: 86%)
Gene names: SAV1345, sbcD
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 11C
Spacegroup: P212121
Unit cell:
a: 73.906Å b: 89.465Å c: 116.575Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.249
Expression system: Escherichia coli BL21(DE3)

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