› 7doj

Solution NMR

Solution structure of TGS domain of the Mycobacterium tuberculosis Rel protein

Released:
DOI: 10.2210/pdb7doj/pdb
PDB entry 7doj coloured by chain, ensemble of 19 models, front view.
PDB entry 7doj coloured by chain, ensemble of 19 models, side view.
PDB entry 7doj coloured by chain, ensemble of 19 models, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Atomic structure of the regulatory TGS domain of Rel protein from Mycobacterium tuberculosis and its interaction with deacylated tRNA.
Shin J, Singal B, Grüber A, Wong DMK, Ragunathan P, Grüber G
FEBS Lett 595 3006-3018 (2021)
PMID: 34808002

Function and Biology Details

Reactions catalysed: 
ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate
Guanosine 3',5'-bis(diphosphate) + H(2)O = guanosine 5'-diphosphate + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161549 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional (p)ppGpp synthase/hydrolase RelA Chain: A
Molecule details ›
Chain: A
Length: 77 amino acids
Theoretical weight: 8.61 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WHG9 (Residues: 394-459; Coverage: 9%)
Gene names: MTCY227.18, Rv2583c, relA
Sequence domains: TGS domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 78%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

The Importance of Microorganisms for Sustainable Agriculture-A Review.
Antoszewski et al. (2022)
1 more