› 7dp4

X-ray diffraction
1.5Å resolution

Crystal structure of wild type Brugia malayi thymidylate synthase complexed with 2'-deoxyuridine monophosphate and methotrexate

Released:
DOI: 10.2210/pdb7dp4/pdb
Model geometry
Fit model/data
PDB entry 7dp4 coloured by chain, front view.
PDB entry 7dp4 coloured by chain, side view.
PDB entry 7dp4 coloured by chain, top view.
Source organism: Brugia malayi
Entry authors: Keyunratsami K, Nualnoi T, Wongkamchai S, Songsiriritthigul C, Chen C-J, Canyuk B

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105142 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate synthase Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.52 KDa
Source organism: Brugia malayi
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1P6CBU1 (Residues: 1-306; Coverage: 100%)
Gene names: BM_BM7277, BM_Bm7277, Bm1_33465, Bm7277, Bma-tyms-1.1
Sequence domains: Thymidylate synthase

Ligands and Environments

3 bound ligands:
Ligand UMP 1 x UMP
Ligand MTX 1 x MTX
Ligand BME 5 x BME
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: P3221
Unit cell:
a: 81.434Å b: 81.434Å c: 105.37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.177 0.191
Expression system: Escherichia coli

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