› 7dr6

Electron Microscopy
4.1Å resolution

PA28alpha-beta in complex with immunoproteasome

Released:
DOI: 10.2210/pdb7dr6/pdb
PDB entry 7dr6 coloured by chain, front view.
PDB entry 7dr6 coloured by chain, side view.
PDB entry 7dr6 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM of mammalian PA28αβ-iCP immunoproteasome reveals a distinct mechanism of proteasome activation by PA28αβ.
Chen J, Wang Y, Xu C, Chen K, Zhao Q, Wang S, Yin Y, Peng C, Ding Z, Cong Y
OpenAccess logo Nat Commun 12 739 (2021)
PMID: 33531497
Related structures: EMD-30824

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 35-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152631 (preferred)
Entry contents:
16 distinct polypeptide molecules
Macromolecules (16 distinct):
Proteasome subunit alpha type-6 Chains: L, e
Molecule details ›
Chains: L, e
Length: 246 amino acids
Theoretical weight: 27.43 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2YDE4 (Residues: 1-246; Coverage: 100%)
Gene name: PSMA6
Sequence domains:
Proteasome subunit alpha type-2 Chains: M, f
Molecule details ›
Chains: M, f
Length: 234 amino acids
Theoretical weight: 25.93 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T0Y5 (Residues: 1-234; Coverage: 100%)
Gene name: PSMA2
Sequence domains:
Proteasome subunit alpha type-4 Chains: N, g
Molecule details ›
Chains: N, g
Length: 261 amino acids
Theoretical weight: 29.53 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3ZCK9 (Residues: 1-261; Coverage: 100%)
Gene name: PSMA4
Sequence domains:
Proteasome subunit alpha type-7 Chains: O, a
Molecule details ›
Chains: O, a
Length: 248 amino acids
Theoretical weight: 27.91 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3ZBG0 (Residues: 1-248; Coverage: 100%)
Gene name: PSMA7
Sequence domains:
Proteasome subunit alpha type-5 Chains: P, b
Molecule details ›
Chains: P, b
Length: 241 amino acids
Theoretical weight: 26.44 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q5E987 (Residues: 1-241; Coverage: 100%)
Gene name: PSMA5
Sequence domains:
Proteasome subunit alpha type-1 Chains: Q, c
Molecule details ›
Chains: Q, c
Length: 263 amino acids
Theoretical weight: 29.63 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T0X5 (Residues: 1-263; Coverage: 100%)
Gene name: PSMA1
Sequence domains:
Proteasome subunit alpha type-3 Chains: R, d
Molecule details ›
Chains: R, d
Length: 255 amino acids
Theoretical weight: 28.44 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q58DU5 (Residues: 1-255; Coverage: 100%)
Gene name: PSMA3
Sequence domains:
Proteasome activator complex subunit 2 Chains: A, D, F
Molecule details ›
Chains: A, D, F
Length: 239 amino acids
Theoretical weight: 27.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UL46 (Residues: 1-239; Coverage: 100%)
Gene name: PSME2
Sequence domains:
Proteasome activator complex subunit 1 Chains: B, C, E, G
Molecule details ›
Chains: B, C, E, G
Length: 249 amino acids
Theoretical weight: 28.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06323 (Residues: 1-249; Coverage: 100%)
Gene names: IFI5111, PSME1
Sequence domains:
Proteasome subunit beta type-3 Chains: H, S
Molecule details ›
Chains: H, S
Length: 205 amino acids
Theoretical weight: 23.02 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P33672 (Residues: 1-205; Coverage: 100%)
Gene name: PSMB3
Sequence domains: Proteasome subunit
Proteasome subunit beta type-2 Chains: I, T
Molecule details ›
Chains: I, T
Length: 201 amino acids
Theoretical weight: 22.92 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q5E9K0 (Residues: 1-201; Coverage: 100%)
Gene name: PSMB2
Sequence domains: Proteasome subunit
Proteasome subunit beta type-1 Chains: J, U
Molecule details ›
Chains: J, U
Length: 241 amino acids
Theoretical weight: 26.28 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2TBX6 (Residues: 1-241; Coverage: 100%)
Gene name: PSMB1
Sequence domains: Proteasome subunit
Proteasome subunit beta type-4 Chains: K, V
Molecule details ›
Chains: K, V
Length: 264 amino acids
Theoretical weight: 29.06 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T108 (Residues: 1-264; Coverage: 100%)
Gene name: PSMB4
Sequence domains: Proteasome subunit
Proteasome subunit beta type-9 Chains: W, Z
Molecule details ›
Chains: W, Z
Length: 219 amino acids
Theoretical weight: 23.43 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3SZC2 (Residues: 1-219; Coverage: 100%)
Gene name: PSMB9
Sequence domains: Proteasome subunit
Proteasome subunit beta type-10 Chains: 1, X
Molecule details ›
Chains: 1, X
Length: 273 amino acids
Theoretical weight: 29.13 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T0T1 (Residues: 1-273; Coverage: 100%)
Gene name: PSMB10
Sequence domains:
Proteasome subunit beta type-8 Chains: 2, Y
Molecule details ›
Chains: 2, Y
Length: 276 amino acids
Theoretical weight: 30.3 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T112 (Residues: 1-276; Coverage: 100%)
Gene name: PSMB8
Sequence domains: Proteasome subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-30824
Expression system: Escherichia coli

Quick links

Citations

4 review citations

PA28γ: New Insights on an Ancient Proteasome Activator.
Cascio P. (2021)
3 more

2 mentions without citation

Structure, Function, and Allosteric Regulation of the 20S Proteasome by the 11S/PA28 Family of Proteasome Activators.
Thomas et al. (2023)
1 more