7drn

X-ray diffraction
3.56Å resolution

Structure of ATP-grasp ligase PsnB complexed with precursor peptide PsnA2 and AMPPNP

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-500776 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP-grasp domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 334 amino acids
Theoretical weight: 37.02 KDa
Source organism: Plesiocystis pacifica SIR-1
Expression system: Escherichia coli
UniProt:
  • Canonical: A6G4D7 (Residues: 1-314; Coverage: 100%)
Gene name: PPSIR1_03893
Sequence domains: RimK-like ATP-grasp domain
PsnA214-38, Precursor peptide Chains: E, F
Molecule details ›
Chains: E, F
Length: 25 amino acids
Theoretical weight: 2.57 KDa
Source organism: Plesiocystis pacifica SIR-1
Expression system: Not provided
UniProt:
  • Canonical: A6GH40 (Residues: 14-38; Coverage: 35%)
Gene name: PPSIR1_14310

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21
Unit cell:
a: 92.294Å b: 92.647Å c: 99.571Å
α: 90° β: 100.9° γ: 90°
R-values:
R R work R free
0.224 0.224 0.228
Expression systems:
  • Escherichia coli
  • Not provided