› 7drw

Electron Microscopy
4.2Å resolution

Bovine 20S immunoproteasome in complex with two human PA28alpha-beta activators

Released:
DOI: 10.2210/pdb7drw/pdb
PDB entry 7drw coloured by chain, front view.
PDB entry 7drw coloured by chain, side view.
PDB entry 7drw coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM of mammalian PA28αβ-iCP immunoproteasome reveals a distinct mechanism of proteasome activation by PA28αβ.
Chen J, Wang Y, Xu C, Chen K, Zhao Q, Wang S, Yin Y, Peng C, Ding Z, Cong Y
OpenAccess logo Nat Commun 12 739 (2021)
PMID: 33531497
Related structures: EMD-30828

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 42-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152632 (preferred)
Entry contents:
16 distinct polypeptide molecules
Macromolecules (16 distinct):
Proteasome subunit alpha type-6 Chains: A, f
Molecule details ›
Chains: A, f
Length: 246 amino acids
Theoretical weight: 27.43 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2YDE4 (Residues: 1-246; Coverage: 100%)
Gene name: PSMA6
Sequence domains:
Proteasome subunit alpha type-2 Chains: B, h
Molecule details ›
Chains: B, h
Length: 234 amino acids
Theoretical weight: 25.93 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T0Y5 (Residues: 1-234; Coverage: 100%)
Gene name: PSMA2
Sequence domains:
Proteasome subunit alpha type-4 Chains: C, j
Molecule details ›
Chains: C, j
Length: 261 amino acids
Theoretical weight: 29.53 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3ZCK9 (Residues: 1-261; Coverage: 100%)
Gene name: PSMA4
Sequence domains:
Proteasome subunit alpha type-7 Chains: D, P
Molecule details ›
Chains: D, P
Length: 248 amino acids
Theoretical weight: 27.91 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3ZBG0 (Residues: 1-248; Coverage: 100%)
Gene name: PSMA7
Sequence domains:
Proteasome subunit alpha type-5 Chains: E, R
Molecule details ›
Chains: E, R
Length: 241 amino acids
Theoretical weight: 26.44 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q5E987 (Residues: 1-241; Coverage: 100%)
Gene name: PSMA5
Sequence domains:
Proteasome subunit alpha type-1 Chains: F, b
Molecule details ›
Chains: F, b
Length: 263 amino acids
Theoretical weight: 29.63 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T0X5 (Residues: 1-263; Coverage: 100%)
Gene name: PSMA1
Sequence domains:
Proteasome subunit alpha type-3 Chains: G, d
Molecule details ›
Chains: G, d
Length: 255 amino acids
Theoretical weight: 28.44 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q58DU5 (Residues: 1-255; Coverage: 100%)
Gene name: PSMA3
Sequence domains:
Proteasome activator complex subunit 2 Chains: H, K, M, O, c, g
Molecule details ›
Chains: H, K, M, O, c, g
Length: 239 amino acids
Theoretical weight: 27.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UL46 (Residues: 1-239; Coverage: 100%)
Gene name: PSME2
Sequence domains:
Proteasome activator complex subunit 1 Chains: I, J, L, N, Q, a, e, i
Molecule details ›
Chains: I, J, L, N, Q, a, e, i
Length: 249 amino acids
Theoretical weight: 28.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06323 (Residues: 1-249; Coverage: 100%)
Gene names: IFI5111, PSME1
Sequence domains:
Proteasome subunit beta type-3 Chains: S, k
Molecule details ›
Chains: S, k
Length: 205 amino acids
Theoretical weight: 23.02 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P33672 (Residues: 1-205; Coverage: 100%)
Gene name: PSMB3
Sequence domains: Proteasome subunit
Proteasome subunit beta type-2 Chains: T, l
Molecule details ›
Chains: T, l
Length: 201 amino acids
Theoretical weight: 22.92 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q5E9K0 (Residues: 1-201; Coverage: 100%)
Gene name: PSMB2
Sequence domains: Proteasome subunit
Proteasome subunit beta type-1 Chains: U, m
Molecule details ›
Chains: U, m
Length: 241 amino acids
Theoretical weight: 26.28 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2TBX6 (Residues: 1-241; Coverage: 100%)
Gene name: PSMB1
Sequence domains: Proteasome subunit
Proteasome subunit beta type-4 Chains: V, n
Molecule details ›
Chains: V, n
Length: 264 amino acids
Theoretical weight: 29.06 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T108 (Residues: 1-264; Coverage: 100%)
Gene name: PSMB4
Sequence domains: Proteasome subunit
Proteasome subunit beta type-9 Chains: W, Z
Molecule details ›
Chains: W, Z
Length: 219 amino acids
Theoretical weight: 23.43 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3SZC2 (Residues: 1-219; Coverage: 100%)
Gene name: PSMB9
Sequence domains: Proteasome subunit
Proteasome subunit beta type-10 Chains: 1, X
Molecule details ›
Chains: 1, X
Length: 273 amino acids
Theoretical weight: 29.13 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T0T1 (Residues: 1-273; Coverage: 100%)
Gene name: PSMB10
Sequence domains:
Proteasome subunit beta type-8 Chains: 2, Y
Molecule details ›
Chains: 2, Y
Length: 276 amino acids
Theoretical weight: 30.3 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T112 (Residues: 1-276; Coverage: 100%)
Gene name: PSMB8
Sequence domains: Proteasome subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-30828
Expression system: Escherichia coli

Quick links

Citations

5 review citations

PA28γ: New Insights on an Ancient Proteasome Activator.
Cascio P. (2021)
4 more

1 mention without citation

Proteasome activator 28γ (PA28γ) allosterically activates trypsin-like proteolysis by binding to the α-ring of the 20S proteasome.
Thomas et al. (2022)