› 7dvl

X-ray diffraction
2.01Å resolution

Crystal Structure of the Catalytic Domain of Botulinum Neurotoxin Subtype A3

Released:
DOI: 10.2210/pdb7dvl/pdb
PDB entry 7dvl coloured by chain, front view.
PDB entry 7dvl coloured by chain, side view.
PDB entry 7dvl coloured by chain, top view.
Source organism: Clostridium botulinum
Primary publication:
Crystal structure of the catalytic domain of botulinum neurotoxin subtype A3.
Leka O, Wu Y, Li X, Kammerer RA
OpenAccess logo J Biol Chem 296 100684
PMID: 33891946

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-505216 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bont/A3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 419 amino acids
Theoretical weight: 47.47 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: D3IV24 (Residues: 1-417; Coverage: 32%)
Gene names: FC871_13445, bont
Sequence domains: Clostridial neurotoxin zinc protease

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 66.25Å b: 97.42Å c: 141.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.232
Expression system: Escherichia coli K-12

Quick links

Citations

1 citations in other articles

A DARPin promotes faster onset of botulinum neurotoxin A1 action.
Leka et al. (2023)

3 mentions without citation

Resolution of Two Steps in Botulinum Neurotoxin Serotype A1 Light Chain Localization to the Intracellular Plasma Membrane.
Gardner et al. (2021)
2 more