› 7dyk

X-ray diffraction
2.99Å resolution

Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC

Released:
DOI: 10.2210/pdb7dyk/pdb
PDB entry 7dyk coloured by chain, front view.
PDB entry 7dyk coloured by chain, side view.
PDB entry 7dyk coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Primary publication:
Elucidation of master allostery essential for circadian clock oscillation in cyanobacteria.
Furuike Y, Mukaiyama A, Ouyang D, Ito-Miwa K, Simon D, Yamashita E, Kondo T, Akiyama S
OpenAccess logo Sci Adv 8 eabm8990 (2022)
PMID: 35427168

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Circadian clock oscillator protein KaiC Chains: A, B
Molecule details ›
Chains: A, B
Length: 519 amino acids
Theoretical weight: 58.07 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79PF4 (Residues: 1-519; Coverage: 100%)
Gene names: Synpcc7942_1216, kaiC, see0011
Sequence domains: KaiC

Ligands and Environments

2 bound ligands:
Ligand ATP 4 x ATP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P63
Unit cell:
a: 94.847Å b: 94.847Å c: 180.56Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.276 0.274 0.328
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Regulation mechanisms of the dual ATPase in KaiC.
Furuike et al. (2022)
3 more