› 7e2y

Electron Microscopy
3Å resolution

Serotonin-bound Serotonin 1A (5-HT1A) receptor-Gi protein complex

Released:
DOI: 10.2210/pdb7e2y/pdb
PDB entry 7e2y coloured by chain, front view.
PDB entry 7e2y coloured by chain, side view.
PDB entry 7e2y coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the lipid and ligand regulation of serotonin receptors.
Xu P, Huang S, Zhang H, Mao C, Zhou XE, Cheng X, Simon IA, Shen DD, Yen HY, Robinson CV, Harpsøe K, Svensson B, Guo J, Jiang H, Gloriam DE, Melcher K, Jiang Y, Zhang Y, Xu HE
Nature 592 469-473 (2021)
PMID: 33762731
Related structures: EMD-30972

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-140422 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.41 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
5-hydroxytryptamine receptor 1A; Soluble cytochrome b562 Chain: R
Molecule details ›
Chain: R
Length: 543 amino acids
Theoretical weight: 60.41 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
  • Canonical: P08908 (Residues: 25-422; Coverage: 94%)
Gene names: ADRB2RL1, ADRBRL1, HTR1A, cybC
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand SRO 1 x SRO
Ligand J40 1 x J40
Ligand CLR 4 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-30972
Expression system: Spodoptera frugiperda

Quick links

Citations

17 review citations

AlphaFold2 versus experimental structures: evaluation on G protein-coupled receptors.
He et al. (2023)
16 more

21 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
20 more