› 7e3f

X-ray diffraction
2.35Å resolution

Crystal structure of Trypanosoma brucei cathepsin B Y217C/S275C mutant

Released:
DOI: 10.2210/pdb7e3f/pdb
PDB entry 7e3f coloured by chain, front view.
PDB entry 7e3f coloured by chain, side view.
PDB entry 7e3f coloured by chain, top view.
Source organism: Trypanosoma brucei brucei TREU927
Primary publication:
Design of an In-Cell Protein Crystal for the Environmentally Responsive Construction of a Supramolecular Filament.
Abe S, Pham TT, Negishi H, Yamashita K, Hirata K, Ueno T
Angew Chem Int Ed Engl 60 12341-12345 (2021)
PMID: 33759310

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112826 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Peptidase C1A papain C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 34.38 KDa
Source organism: Trypanosoma brucei brucei TREU927
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: D6XHE1 (Residues: 27-338; Coverage: 98%)
Gene names: Tb06.3D8.230, Tb927.6.560
Sequence domains: Papain family cysteine protease

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P42212
Unit cell:
a: 123.65Å b: 123.65Å c: 54.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.257
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

Protein Assembly by Design.
Zhu et al. (2021)
3 other articles