› 7e4b

X-ray diffraction
1.77Å resolution

Crystal structure of MIF bound to compound 5

Released:
DOI: 10.2210/pdb7e4b/pdb
PDB entry 7e4b coloured by chain, front view.
PDB entry 7e4b coloured by chain, side view.
PDB entry 7e4b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification and Structure-Activity Relationships of Dietary Flavonoids as Human Macrophage Migration Inhibitory Factor (MIF) Inhibitors.
Yang L, Guo D, Fan C
J Agric Food Chem 69 10138-10150 (2021)
PMID: 34459191

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 114 amino acids
Theoretical weight: 12.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14174 (Residues: 2-115; Coverage: 99%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)

Ligands and Environments

2 bound ligands:
Ligand CL 4 x CL
Ligand MRI 1 x MRI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 66.028Å b: 68.513Å c: 85.333Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.232
Expression system: Escherichia coli

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Citations

1 citations in other articles

The activation of PPARγ enhances Treg responses through up-regulating CD36/CPT1-mediated fatty acid oxidation and subsequent N-glycan branching of TβRII/IL-2Rα.
Miao et al. (2022)