› 7e51

Electron Microscopy
3.23Å resolution

Structure of PEP bound Enolase from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb7e51/pdb
PDB entry 7e51 coloured by chain, front view.
PDB entry 7e51 coloured by chain, side view.
PDB entry 7e51 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structural snapshots of Mycobacterium tuberculosis enolase reveal dual mode of 2PG binding and its implication in enzyme catalysis.
Ahmad M, Jha B, Bose S, Tiwari S, Dwivedy A, Kar D, Pal R, Mariadasse R, Parish T, Jeyakanthan J, Vinothkumar KR, Biswal BK
OpenAccess logo IUCrJ 10 738-753 (2023)
PMID: 37860976
Related structures: EMD-30989

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161866 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enolase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 436 amino acids
Theoretical weight: 45.96 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Mycolicibacterium smegmatis
UniProt:
  • Canonical: P9WNL1 (Residues: 1-429; Coverage: 100%)
Gene names: MTCY10G2.26c, Rv1023, eno
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand PEP 8 x PEP
Ligand MG 16 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.23Å
Relevant EMDB volumes: EMD-30989
Expression system: Mycolicibacterium smegmatis

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Citations

1 mention without citation

Structural snapshots of Mycobacterium tuberculosis enolase reveal dual mode of 2PG binding and its implication in enzyme catalysis.
Ahmad et al. (2023)