› 7e6b

X-ray diffraction
1.84Å resolution

Crystal structure of PMP-bound form of cysteine desulfurase SufS C361A from Bacillus subtilis

Released:
DOI: 10.2210/pdb7e6b/pdb
PDB entry 7e6b coloured by chain, front view.
PDB entry 7e6b coloured by chain, side view.
PDB entry 7e6b coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Cycloserine enantiomers inhibit PLP-dependent cysteine desulfurase SufS via distinct mechanisms.
Nakamura R, Ogawa S, Takahashi Y, Fujishiro T
FEBS J (2022)
PMID: 35395703

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase SufS Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 46.55 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32164 (Residues: 1-406; Coverage: 100%)
Gene names: BSU32690, csd, sufS, yurW
Sequence domains: Aminotransferase class-V

Ligands and Environments


Cofactor: Ligand PMP 1 x PMP
3 bound ligands:
Ligand EDO 3 x EDO
Ligand PEG 7 x PEG
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P3121
Unit cell:
a: 92.8Å b: 92.8Å c: 129Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.157 0.178
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

Exploring the mechanism of Alisma orientale for the treatment of pregnancy induced hypertension and potential hepato-nephrotoxicity by using network pharmacology, network toxicology, molecular docking and molecular dynamics simulation.
Liao et al. (2022)
1 more

1 mention without citation

Cycloserine enantiomers inhibit PLP-dependent cysteine desulfurase SufS via distinct mechanisms.
Nakamura et al. (2022)