Function and Biology Details
Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
Non-structural protein 3 (preferred)
PDBe Complex ID:
PDB-CPX-143068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3C-like proteinase
Molecule details ›
Chains: A, B
Length: 303 amino acids
Theoretical weight: 32.76 KDa
Source organism: Human coronavirus NL63
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: Coronavirus endopeptidase C30
Length: 303 amino acids
Theoretical weight: 32.76 KDa
Source organism: Human coronavirus NL63
Expression system: Escherichia coli BL21
UniProt:
- Canonical:
P0C6U6 (Residues: 2940-3242; Coverage: 8%)
Sequence domains: Coronavirus endopeptidase C30
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SSRF BEAMLINE BL17U1
Spacegroup:
P21
Expression system: Escherichia coli BL21
