› 7e8t

Electron Microscopy
3.8Å resolution

Monomer of Ypt32-TRAPPII

Released:
DOI: 10.2210/pdb7e8t/pdb
PDB entry 7e8t coloured by chain, front view.
PDB entry 7e8t coloured by chain, side view.
PDB entry 7e8t coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis for assembly of TRAPPII complex and specific activation of GTPase Ypt31/32.
Mi C, Zhang L, Huang G, Shao G, Yang F, You X, Dong MQ, Sun S, Sui SF
OpenAccess logo Sci Adv 8 eabi5603 (2022)
PMID: 35080977
Related structures: EMD-31022

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152400 (preferred)
Entry contents:
11 distinct polypeptide molecules
Macromolecules (11 distinct):
GTP-binding protein YPT32/YPT11 Chain: L
Molecule details ›
Chain: L
Length: 222 amino acids
Theoretical weight: 24.55 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P51996 (Residues: 1-222; Coverage: 100%)
Gene names: YGL210W, YPT11, YPT32
Sequence domains: Ras family
Trafficking protein particle complex II-specific subunit 130 Chain: I
Molecule details ›
Chain: I
Length: 1102 amino acids
Theoretical weight: 128.23 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03660 (Residues: 1-1102; Coverage: 100%)
Gene names: TRS130, YM8261.12C, YMR218C
Sequence domains: Trafficking protein particle complex subunit 10, TRAPPC10
Trafficking protein particle complex II-specific subunit 120 Chain: J
Molecule details ›
Chain: J
Length: 1289 amino acids
Theoretical weight: 147.82 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q04183 (Residues: 1-1289; Coverage: 100%)
Gene names: D9509.25, ESBP10, TRS120, YDR407C
Sequence domains: Transport protein Trs120 or TRAPPC9, TRAPP II complex subunit
Trafficking protein particle complex II-specific subunit 65 Chain: K
Molecule details ›
Chain: K
Length: 560 amino acids
Theoretical weight: 63.43 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32893 (Residues: 1-560; Coverage: 100%)
Gene names: KRE11, TRS65, YGR166W
Sequence domains: TRAPP trafficking subunit Trs65
Trafficking protein particle complex subunit 33 Chain: B
Molecule details ›
Chain: B
Length: 268 amino acids
Theoretical weight: 30.79 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q99394 (Residues: 1-268; Coverage: 100%)
Gene names: O3251, TRS33, YOR115C, YOR3251C
Sequence domains: Transport protein particle (TRAPP) component
Trafficking protein particle complex subunit 31 Chain: G
Molecule details ›
Chain: G
Length: 283 amino acids
Theoretical weight: 31.74 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03337 (Residues: 1-283; Coverage: 100%)
Gene names: TRS31, YDR472W
Sequence domains: Transport protein particle (TRAPP) component
Trafficking protein particle complex subunit 23 Chain: E
Molecule details ›
Chain: E
Length: 219 amino acids
Theoretical weight: 24.89 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03784 (Residues: 1-219; Coverage: 100%)
Gene names: TRS23, YD8419.13, YDR246W
Sequence domains: Sybindin-like family
Trafficking protein particle complex subunit 20 Chain: H
Molecule details ›
Chain: H
Length: 175 amino acids
Theoretical weight: 19.72 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38334 (Residues: 1-175; Coverage: 100%)
Gene names: TRS20, YBR1722, YBR254C
Sequence domains: Sedlin, N-terminal conserved region
TRAPP-associated protein TCA17 Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 17.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32613 (Residues: 1-152; Coverage: 100%)
Gene names: SYGP-ORF36, TCA17, YEL048C
Sequence domains: Sedlin, N-terminal conserved region
Trafficking protein particle complex subunit BET5 Chain: D
Molecule details ›
Chain: D
Length: 159 amino acids
Theoretical weight: 18.45 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03630 (Residues: 1-159; Coverage: 100%)
Gene names: BET5, YML077W
Sequence domains: Sybindin-like family
Trafficking protein particle complex subunit BET3 Chains: C, F
Molecule details ›
Chains: C, F
Length: 193 amino acids
Theoretical weight: 22.15 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P36149 (Residues: 1-193; Coverage: 100%)
Gene names: BET3, YKR068C
Sequence domains: Transport protein particle (TRAPP) component

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-31022
Expression system: Saccharomyces cerevisiae

Quick links

Citations

2 review citations

The TRAPP complexes: oligomeric exchange factors that activate the small GTPases Rab1 and Rab11.
Galindo et al. (2023)
1 more

1 mention without citation

The eukaryotic-like characteristics of small GTPase, roadblock and TRAPPC3 proteins from Asgard archaea.
Tran et al. (2024)