› 7ea2

X-ray diffraction
2.14Å resolution

crystal structure of NAP1 FIR in complex with RB1CC1 Claw domain

Released:
DOI: 10.2210/pdb7ea2/pdb
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PDB entry 7ea2 coloured by chain, front view.
PDB entry 7ea2 coloured by chain, side view.
PDB entry 7ea2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and biochemical advances on the recruitment of the autophagy-initiating ULK and TBK1 complexes by autophagy receptor NDP52.
Fu T, Zhang M, Zhou Z, Wu P, Peng C, Wang Y, Gong X, Li Y, Wang Y, Xu X, Li M, Shen L, Pan L
OpenAccess logo Sci Adv 7 (2021)
PMID: 34389544

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186220 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-azacytidine-induced protein 2; RB1-inducible coiled-coil protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9H6S1 (Residues: 6-16; Coverage: 3%)
  • Canonical: Q8TDY2 (Residues: 1490-1594; Coverage: 7%)
Gene names: AZI2, KIAA0203, NAP1, RB1CC1, RBICC, TBKBP2
Sequence domains: Autophagy-related protein 11

Ligands and Environments

3 bound ligands:
Ligand P6G 1 x P6G
Ligand PEG 2 x PEG
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P6122
Unit cell:
a: 67.62Å b: 67.62Å c: 247.37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.232
Expression system: Escherichia coli BL21

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Citations

2 review citations

The mechanisms and roles of selective autophagy in mammals.
Vargas et al. (2023)
1 more

1 mention without citation

Mechanisms underlying ubiquitin-driven selective mitochondrial and bacterial autophagy.
Goodall et al. (2022)

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