› 7ebe

X-ray diffraction
2.69Å resolution

Crystal structure of Isocitrate lyase-1 from Candida albicans

Released:
DOI: 10.2210/pdb7ebe/pdb
Model geometry
Fit model/data
PDB entry 7ebe coloured by chain, front view.
PDB entry 7ebe coloured by chain, side view.
PDB entry 7ebe coloured by chain, top view.
Source organism: Candida albicans
Primary publication:
Structural insights into the targeting specificity of ubiquitin ligase for S. cerevisiae isocitrate lyase but not C. albicans isocitrate lyase.
Hiragi K, Nishio K, Moriyama S, Hamaguchi T, Mizoguchi A, Yonekura K, Tani K, Mizushima T
J Struct Biol 213 107748 (2021)
PMID: 34033899

Function and Biology Details

Reactions catalysed: 
Isocitrate = succinate + glyoxylate
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192871 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate lyase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 556 amino acids
Theoretical weight: 62.34 KDa
Source organism: Candida albicans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9P8Q7 (Residues: 1-550; Coverage: 100%)
Gene name: ICL1
Sequence domains: Isocitrate lyase family

Ligands and Environments

2 bound ligands:
Ligand MG 8 x MG
Ligand FMT 12 x FMT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 80.971Å b: 139.91Å c: 200.301Å
α: 90° β: 92.55° γ: 90°
R-values:
R R work R free
0.216 0.215 0.248
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Study of peripheral domains in structure-function of isocitrate lyase (ICL) from Pseudomonas aeruginosa.
Díaz-Pérez et al. (2023)