› 7ec5

Electron Microscopy
2.89Å resolution

EV-D68 in complex with 8F12 Fab

Released:
DOI: 10.2210/pdb7ec5/pdb
PDB entry 7ec5 coloured by chain, front view.
PDB entry 7ec5 coloured by chain, side view.
PDB entry 7ec5 coloured by chain, top view.
Source organisms:
Primary publication:
Functional and structural characterization of a two-MAb cocktail for delayed treatment of enterovirus D68 infections.
Zhang C, Xu C, Dai W, Wang Y, Liu Z, Zhang X, Wang X, Wang H, Gong S, Cong Y, Huang Z
OpenAccess logo Nat Commun 12 2904 (2021)
PMID: 34006855
Related structures: EMD-31056

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 360-mer (preferred)
PDBe Complex ID:
PDB-CPX-278909 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
8F12 Fab light chain Chain: F
Molecule details ›
Chain: F
Length: 213 amino acids
Theoretical weight: 23.38 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
8F12 Fab heavy chain Chain: E
Molecule details ›
Chain: E
Length: 216 amino acids
Theoretical weight: 23.23 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Capsid protein VP1 Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 32.92 KDa
Source organism: enterovirus D68
UniProt:
  • Canonical: A0A097BW12 (Residues: 565-861; Coverage: 14%)
Sequence domains: Picornavirus coat protein
Capsid protein VP3 Chain: C
Molecule details ›
Chain: C
Length: 247 amino acids
Theoretical weight: 27.11 KDa
Source organism: enterovirus D68
UniProt:
  • Canonical: A0A097BW12 (Residues: 318-564; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Genome polyprotein Chain: B
Molecule details ›
Chain: B
Length: 248 amino acids
Theoretical weight: 27.57 KDa
Source organism: enterovirus D68
UniProt:
  • Canonical: A0A097BW12 (Residues: 70-317; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Capsid protein VP4 Chain: D
Molecule details ›
Chain: D
Length: 68 amino acids
Theoretical weight: 7.34 KDa
Source organism: enterovirus D68
UniProt:
  • Canonical: J9Z449 (Residues: 2-69; Coverage: 47%)
Sequence domains: Picornavirus coat protein (VP4)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.89Å
Relevant EMDB volumes: EMD-31056
Expression system: Homo sapiens

Quick links

Citations

1 review citation

From the "One-Molecule, One-Target, One-Disease" Concept towards Looking for Multi-Target Therapeutics for Treating Non-Polio Enterovirus (NPEV) Infections.
Roux et al. (2024)
7 other articles