› 7efq

X-ray diffraction
2.3Å resolution

Crystal structure of hPPARgamma ligand binding domain complexed with rosiglitazone-based fluorescence probe

Released:
DOI: 10.2210/pdb7efq/pdb
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PDB entry 7efq coloured by chain, front view.
PDB entry 7efq coloured by chain, side view.
PDB entry 7efq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Synthesis of a Coumarin-Based PPARγ Fluorescence Probe for Competitive Binding Assay.
Yoshikawa C, Ishida H, Ohashi N, Itoh T
OpenAccess logo Int J Mol Sci 22 (2021)
PMID: 33919837

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisome proliferator-activated receptor gamma Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 31.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli #1/H766
UniProt:
  • Canonical: P37231 (Residues: 232-505; Coverage: 54%)
Gene names: NR1C3, PPARG
Sequence domains: Ligand-binding domain of nuclear hormone receptor

Ligands and Environments

1 bound ligand:
Ligand J3F 1 x J3F
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 93.33Å b: 61.9Å c: 119.37Å
α: 90° β: 102.29° γ: 90°
R-values:
R R work R free
0.226 0.224 0.264
Expression system: Escherichia coli #1/H766

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Citations

1 review citation

Heat Shock Protein 90 (HSP90) Inhibitors as Anticancer Medicines: A Review on the Computer-Aided Drug Discovery Approaches over the Past Five Years.
Magwenyane et al. (2022)
2 other articles

1 mention without citation

Synthesis of a Coumarin-Based PPARγ Fluorescence Probe for Competitive Binding Assay.
Yoshikawa et al. (2021)

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