› 7ehf

X-ray diffraction
1.5Å resolution

Crystal structure of the aminoglycoside resistance methyltransferase NpmB1

Released:
DOI: 10.2210/pdb7ehf/pdb
PDB entry 7ehf coloured by chain, front view.
PDB entry 7ehf coloured by chain, side view.
PDB entry 7ehf coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Functional and Structural Characterization of Acquired 16S rRNA Methyltransferase NpmB1 Conferring Pan-Aminoglycoside Resistance.
Kawai A, Suzuki M, Tsukamoto K, Minato Y, Doi Y
Antimicrob Agents Chemother 65 e0100921 (2021)
PMID: 34310216

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168220 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
16S rRNA methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 23.86 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand CL 1 x CL
Ligand EDO 7 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 42.738Å b: 65.798Å c: 65.806Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.194
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Research Updates of Plasmid-Mediated Aminoglycoside Resistance 16S rRNA Methyltransferase.
Yang et al. (2022)
1 more