› 7eht

X-ray diffraction
1.45Å resolution

Levansucrase from Brenneria sp. EniD 312

Released:
DOI: 10.2210/pdb7eht/pdb
PDB entry 7eht coloured by chain, front view.
PDB entry 7eht coloured by chain, side view.
PDB entry 7eht coloured by chain, top view.
Source organism: Brenneria sp. EniD312
Primary publication:
Crystal Structure of Levansucrase from the Gram-Negative Bacterium Brenneria Provides Insights into Its Product Size Specificity.
Xu W, Ni D, Hou X, Pijning T, Guskov A, Rao Y, Mu W
J Agric Food Chem 70 5095-5105 (2022)
PMID: 35388691

Function and Biology Details

Reaction catalysed: 
Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
levansucrase Chain: A
Molecule details ›
Chain: A
Length: 443 amino acids
Theoretical weight: 49.33 KDa
Source organism: Brenneria sp. EniD312
Expression system: Escherichia coli
UniProt:
  • Canonical: G7LSK3 (Residues: 1-437; Coverage: 100%)
Gene name: BrE312_3941
Sequence domains: Levansucrase/Invertase

Ligands and Environments

4 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 8 x GOL
Ligand P33 2 x P33
Ligand PGE 4 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P6122
Unit cell:
a: 97.974Å b: 97.974Å c: 214.22Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.172 0.19
Expression system: Escherichia coli

Quick links