› 7eiu

X-ray diffraction
2.35Å resolution

Crystal structure of Mei2 RRM3 in complex with 8mer meiRNA

Released:
DOI: 10.2210/pdb7eiu/pdb
PDB entry 7eiu coloured by chain, front view.
PDB entry 7eiu coloured by chain, side view.
PDB entry 7eiu coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights reveal the specific recognition of meiRNA by the Mei2 protein.
Shen S, Jian Y, Cai Z, Li F, Lv M, Liu Y, Wu J, Fu C, Shi Y
OpenAccess logo J Mol Cell Biol (2022)
PMID: 35512546

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140451 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Meiosis protein mei2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 155 amino acids
Theoretical weight: 17.99 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: P08965 (Residues: 580-733; Coverage: 21%)
Gene names: SPAC27D7.03c, mei2
Sequence domains: RNA recognition motif 2
RNA (5'-R(P*UP*UP*CP*UP*GP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 nucleotides
Theoretical weight: 1.83 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2221
Unit cell:
a: 66.227Å b: 162.119Å c: 96.657Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.225
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Thirty Years with ERH: An mRNA Splicing and Mitosis Factor Only or Rather a Novel Genome Integrity Protector?
Kozlowski P. (2023)

1 mention without citation

Structural insights reveal the specific recognition of meiRNA by the Mei2 protein.
Shen et al. (2022)