Function and Biology Details
Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- P-loop containing nucleoside triphosphate hydrolase
- Nonstructural protein 13, zinc-binding domain, coronavirus-like
- Non-structural protein NSP8 superfamily, coronavirus
- Non-structural protein NSP8, coronavirus
- RNA synthesis protein NSP10, coronavirus
- Non-structural protein NSP9 superfamily, coronavirus
- DNA2/NAM7 helicase-like, C-terminal
- (+) RNA virus helicase core domain
16 more domains
Structure analysis Details
Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144131 (preferred)
Entry contents:
7 distinct polypeptide molecules
2 distinct RNA molecules
2 distinct RNA molecules
Macromolecules (9 distinct):
RNA-directed RNA polymerase nsp12
Molecule details ›
Chain: A
Length: 929 amino acids
Theoretical weight: 106.44 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 929 amino acids
Theoretical weight: 106.44 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P0DTD1 (Residues: 4393-5321; Coverage: 13%)
Sequence domains:
Non-structural protein 8
Molecule details ›
Chains: B, D
Length: 198 amino acids
Theoretical weight: 21.9 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Coronavirus replicase NSP8
Length: 198 amino acids
Theoretical weight: 21.9 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P0DTD1 (Residues: 3943-4140; Coverage: 3%)
Sequence domains: Coronavirus replicase NSP8
Non-structural protein 7
Molecule details ›
Chain: C
Length: 83 amino acids
Theoretical weight: 9.25 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
Length: 83 amino acids
Theoretical weight: 9.25 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P0DTC1 (Residues: 3860-3942; Coverage: 2%)
Viral protein genome-linked nsp9
Molecule details ›
Chain: G
Length: 113 amino acids
Theoretical weight: 12.39 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Coronavirus replicase NSP9
Length: 113 amino acids
Theoretical weight: 12.39 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P0DTD1 (Residues: 4141-4253; Coverage: 2%)
Sequence domains: Coronavirus replicase NSP9
Non-structural protein 10
Molecule details ›
Chain: H
Length: 139 amino acids
Theoretical weight: 14.8 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Coronavirus RNA synthesis protein NSP10
Length: 139 amino acids
Theoretical weight: 14.8 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P0DTD1 (Residues: 4254-4392; Coverage: 2%)
Sequence domains: Coronavirus RNA synthesis protein NSP10
Guanine-N7 methyltransferase nsp14
Molecule details ›
Chain: K
Length: 527 amino acids
Theoretical weight: 59.89 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Coronavirus proofreading exoribonuclease
Length: 527 amino acids
Theoretical weight: 59.89 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P0DTD1 (Residues: 5926-6452; Coverage: 7%)
Sequence domains: Coronavirus proofreading exoribonuclease
Helicase nsp13
Molecule details ›
Chains: E, F
Length: 601 amino acids
Theoretical weight: 66.93 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 601 amino acids
Theoretical weight: 66.93 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P0DTD1 (Residues: 5325-5925; Coverage: 9%)
Sequence domains:
