› 7ej0

Electron Microscopy
3.2Å resolution

Structure of the alpha2A-adrenergic receptor GoA signaling complex

Released:
DOI: 10.2210/pdb7ej0/pdb
PDB entry 7ej0 coloured by chain, front view.
PDB entry 7ej0 coloured by chain, side view.
PDB entry 7ej0 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into ligand recognition, activation, and signaling of the α2A adrenergic receptor.
Xu J, Cao S, Hübner H, Weikert D, Chen G, Lu Q, Yuan D, Gmeiner P, Liu Z, Du Y
OpenAccess logo Sci Adv 8 eabj5347 (2022)
PMID: 35245122
Related structures: EMD-31147

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225110 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(o) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.1 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P09471 (Residues: 1-354; Coverage: 100%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 349 amino acids
Theoretical weight: 38.4 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: H
Molecule details ›
Chain: H
Length: 307 amino acids
Theoretical weight: 32.9 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Alpha-2A adrenergic receptor Chain: R
Molecule details ›
Chain: R
Length: 465 amino acids
Theoretical weight: 50.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08913 (Residues: 1-465; Coverage: 100%)
Gene names: ADRA2A, ADRA2R, ADRAR
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

1 bound ligand:
Ligand E5E 1 x E5E
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-31147
Expression systems:
  • Trichoplusia ni
  • Spodoptera frugiperda

Quick links

Citations

1 review citation

Signalling of Adrenoceptors: Canonical Pathways and New Paradigms.
Mastos et al. (2024)
8 other articles

5 mentions without citation

A Novel "Activation Switch" Motif Common to All Aminergic Receptors.
Lee et al. (2023)
4 more