› 7ek8

X-ray diffraction
1.7Å resolution

Crystal structure of apo streptavidin at ambient temperature

Released:
DOI: 10.2210/pdb7ek8/pdb
PDB entry 7ek8 coloured by chain, front view.
PDB entry 7ek8 coloured by chain, side view.
PDB entry 7ek8 coloured by chain, top view.
Source organism: Streptomyces avidinii
Entry authors: DeMirci H, Ertem FB, Destan E, Ayan E

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 123 amino acids
Theoretical weight: 12.98 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 38-160; Coverage: 77%)
Sequence domains: Avidin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE MFX
Spacegroup: P21
Unit cell:
a: 47.4Å b: 87.7Å c: 58.9Å
α: 90° β: 98.9° γ: 90°
R-values:
R R work R free
0.192 0.19 0.224
Expression system: Escherichia coli

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